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还沉浸于传统的基因克隆方法无法自拔吗?

        “从一开始我采用的就是传统的基因克隆方法,而且每个人
都在用。因此做克隆总是会想到连接酶!”
艾克隆
2011届硕士研究生

是时候改变自己的习惯了!


In-Fusion HD Cloning
Plus Systems


PCR克隆试剂盒——采用In-Fusion HD Cloning Plus进行高效DNA无缝克隆

        一款简便高效、不再需要连接酶的DNA无缝克隆试剂盒,打破传统思维,注入基因克隆的新鲜血液。
Learn more now
  • 忘掉连接酶吧:打破传统连接酶的应用,新颖!
  • 忘掉内切酶吧:酶切位点随意选,轻松!
  • 忘掉小捣蛋吧:冗余碱基不再有,靠谱!
  • 忘掉亚克隆吧:多片段克隆一步到位,给力!
多片段克隆
一步到位,无需亚克隆



点突变
缺失、添加或者替换——一个试剂盒全部搞定

高通量克隆
        Oxford Protein Production Facility(OPPF)已采用Clontech In-Fusion 克隆系统构建了pOPIN vector 重组载体库 。OPPF科学家以703个PCR片段为目的片段成功构建了661重组载体,总体克隆效率可达到94%。文章已发表在Nucleic Acids Research上。

        获得更多clontech资讯请登录官方网站www.clontech.com或者关注Clontech微博http://weibo.com/clontechchina

“I’m In-fusion, I forgot cloning”

 
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