p38 MAPK (Thr180, Tyr182) 磷酸化兔单抗免费试用

义翘神州通过自有的流式单B技术制备了一系列磷酸化兔单抗。磷酸化兔单抗经过多种实验技术验证其特异性，应用广泛，有效保证了抗体的批间差、稳定性。

p38是丝裂原活化蛋白激酶MAPK家族的重要成员。p38的激活需要Thr180、Tyr182位点发生磷酸化，在细胞增殖、代谢、凋亡等方面发挥重要作用，与多种肿瘤发展相关。

义翘神州通过其自有的流式单B技术制备了一系列磷酸化兔单抗。磷酸化兔单抗经过多种实验技术验证其特异性，应用广泛，有效保证了抗体的批间差、稳定性。

下面以Recombinant Phospho-p38 MAPK (Thr180, Tyr182) Antibody, Rabbit Monoclonal （Cat: 110435-R0004）为例为大家介绍：

• WB应用（多肽竞争特异性验证）

Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using Phospho-p38 MAPK (Thr180, Tyr182) rabbit monoclonal Antibody (Cat# 110435-R0004) at 1:1000 dilution.

• IHC应用（磷酸酶特异性验证）

Immunohistochemical analysis of paraffin-embedded human carcinoma of sigmoid tissue,untreated (left) or lambda phosphatase-treated (right), using Recombinant Phospho-p38 MAPK (Thr180-Tyr182) Antibody, Rabbit Monoclonal (Cat# 110435-R0004) at 1:500 dilution.

• IF应用（磷酸酶特异性验证）

Immunofluorescence staining of Phospho-p38 MAPK (Thr180, Tyr182) in serum-starved NIH-3T3 cells, untreated (left), treated with UV (30mins) (middle) or treated with UV (30mins) and lambda phosphatase (right). Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Mouse Phospho-p38 MAPK (Thr180, Tyr182) monoclonal antibody (dilution ratio 1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was mainly localized to Nucleus.

• 稳定性测试（加速实验）

Recombinant Phospho-p38 MAPK (Thr180, Tyr182) Antibody, Rabbit Monoclonal (Cat: 110435-R0004) was placed in 25℃ for 8 days, in 25℃ for 16 days, in 37℃ for 3.5 days, in 37℃ for 7 days respectively. The cell lysates were serum-starved NIH-3T3, untreated or treated with UV (30 min). The antibody after accelerated by different temperature still showed significant specificity.

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