周俭民研究员miRNA调节研究取得新进展

　　

生物通报道，北京生命科学研究所周俭民实验室在《Plant Physiology》杂志在线发表题为“Identification of miRNAs involved in Pathogen-Associated Molecular Pattern-triggered plant innate immunity”的文章，该文报道了病原菌的PAMP分子flg22可以通过调节与Argonaute(AGO1)蛋白结合的miRNA的累积来调节PAMP激活的先天免疫反应。

 

研究员周俭民博士是文本的通讯作者。此项研究为科技部863和北京市科委资助课题，在北京生命科学研究所完成。

 

在这篇论文中周俭民研究组系统分析了与miRNA和siRNA信号途径相关的调节因子AGO1的抗病表型，发现AGO1 正向调节PAMP诱导的胼胝质沉积，抗性相关基因转录，植物幼苗生长抑制和flg22诱导的植物抗病性。

 

通过对flg22诱导条件下与AGO1结合的小RNA进行全面测序分析发现，flg22调节部分与AGO1结合的miRNA的累积水平。对这些受调控的miRNA的目的基因进行real-time PCR分析发现，受flg22诱导累积上调的miR158和miR160的目的基因在flg22诱导条件下转录降低，受Flg22 诱导累积下降的miR398和miR773的目的基因在flg22诱导条件下转录增加，暗示flg22通过调节miRNA的累积来调控其目的基因的转录。在miR160 过表达植物中PAMP诱导的胼胝质沉积有所增加；而miR398和miR773过表达植物上，PAMP诱导的胼胝质沉积明显少于野生型，对毒性菌DC3000和非毒性菌DC3000(hrcC-)也更敏感。我们认为 flg22 通过调节与AGO1结合的miRNA的累积来调控与植物抗病相关的目的基因的转录，从而调节植物对细菌的先天免疫。

（生物通 小茜）

生物通推荐原文检索

Identification of miRNAs involved in Pathogen-Associated Molecular Pattern-triggered plant innate immunity

 

周俭民

北京生命科学研究所研究员

实验室的主要研究兴趣是植物与病原微生物间相互作用的机理。植物可以感受病原菌的入侵，并且启动防卫反应成功地抵御病原菌的感染。而病原菌通过适应寄主产生相应的毒性机制感染寄主，引发病害。我们用拟南芥和假单孢杆菌间互作为模式回答植物的抗病机理和病原菌的致病机理。目前的工作主要回答植物是如何识别细菌并激活先天免疫的信号传导通路，细菌致病蛋白的生化功能及在寄主中的靶点等问题。对这些问题的回答，有利于农业生产中对病害的控制。另外，因为动植物先天免疫机理的相似性和植物遗传易操作性，我们的工作还有助对动物先天免疫的认识。

Publications:

1. Na Zong, Tingting Xiang, Yan Zou, Jijie Chai, and Jian-Min Zhou (2008). Blocking and triggering of plant immunity by Pseudomonas syringae effector AvrPto. Plant Signaling & Behavior, 3: 583-585.

 2. Jian-Min Zhou and Jijie Chai (2008). Plant pathogenic bacterial effectors subdue host responses. Current Opinion in Microbiology, 11: 179-185 (Invited Review).

 3. Huamin Chen, Yan Zou, Yulei Shang, Huiqiong Lin, Yujing Wang, Run Cai, Xiaoyan Tang, and Jian-Min Zhou (2008). Firefly luciferase complementation imaging assay for protein-protein interactions in plants. Plant Physiol. 146: 368-376.

 4. Linjie Chen, Huayi Wang, Jie Zhang, Lichuan Gu, Niu Huang, Jian-Min Zhou, and Jijie Chai (2008). Structural basis for the catalytic mechanism of phosphothreonine lyase. Nat Struct Mol Biol, 15, 101-102.

 5. Tingting Xiang, Na Zong, Yan Zou, Yong Wu, Jie Zhang, Weiman Xing, Yan Li, Xiaoyan Tang, Lihuang Zhu, Jijie Chai, and Jian-Min Zhou (2008). Pseudomonas syringae effector AvrPto blocks innate immunity by targeting receptor kinases. Curr Biol, 18, 74-80.

 6. Weiman Xing, Yan Zou, Qun Liu, Jianing Liu, Xi Luo, Qingqiu Huang, She Chen, Lihuang Zhu, Ruchang Bi, Quan Hao, Jia-Wei Wu, Jian-Min Zhou, and Jijie Chai (2007). Structural basis for activation of plant immunity by bacterial effector protein AvrPto. Nature, 449, 243-247.

 7. Jie Zhang, Feng Shao, Yan Li, Haitao Cui, Linjie Chen, Hongtao Li, Yan Zou, Chengzu Long, Lefu Lan, Jijie Chai, She Chen, Xiaoyan Tang, and Jian-Min Zhou (2007). A Pseudomonas syringae Effector Inactivates MAPKs to Suppress PAMP-Induced Immunity. Cell Host & Microbe, 1,175-185.

 8. Hongtao Li, Hao Xu, Yan Zhou, Jie Zhang, Chengzu Long, Shuqin Li, She Chen, Jian-Min Zhou, Feng Shao (2007). The phosphothreonine lyase activity of a bacterial type III effector family. Science 315: 1000-1003.

 9. Yanmei Xiao, Lefu Lan, Chuntao Yin, Xin Deng, Doug W. Baker, Jian-Min Zhou, Xiaoyan Tang (2007). Two-component sensor RhpS promotes induction of Pseudomonas syringae type III secretion system by repressing negative regulator RhpR. MPMI, 20: 223-234.

 10. Lefu Lan, Xin Deng, Yanmei Xiao, Jian-Min Zhou, Xiaoyan Tang (2007). Mutation of Lon protease differentially affects Pseudomonas syringae TTSS gene expression in rich and minimal media and reduces pathogenicity. MPMI, 20: 682-696.