Nature子刊专题：新一代测序技术（免费）

　　

生物通报道：DNA测序方法起始于英国生物化学家桑格（F. Sanger）在1975年建立的“加减法”，之后美国生物化学家马利斯（K.B. Mullis）发明了聚合酶链反应PCR技术，被迅速的应用于测序，大大的提高了测序的速度。经过30年的发展，DNA测序由半自动化发展到全部仪器自动测序，但基本的原理仍然没有完全跳出Sanger测序方法。

在2005年454公司推出了比Sanger测序方法快100倍的新型测序仪，推广了Pyrosequencing测序方法，从而引发了测序市场比拼的高潮。近期ABI公司又推出了SOLiD 3系统，并应用这一系统的寡核苷酸连接和检测技术对人类基因组进行了测序，费用低于6万美元。这台新仪器的技术改进使更高的样品和数据通量成为可能，从而有望进一步降低基因组测序的费用。

下一代测序技术到底会怎么样呢，08年10月《Nature Biotechnology》就以Next-Generation Sequencing（下一代测序技术）为题进行了专题报道。其中介绍了大型测序技术的现状，以及这些平台如何改变了生物研究，生物医药和测序市场的面貌，这些相关文章都是免费下载的（到09年4月截止）。

社论：

Prepare for the deluge
doi:10.1038/nbt1008-1099

The gobs of data produced by next-generation sequencing are a key problem limiting wider adoption.
 

新闻：

Profile: Rade Drmanac
Laura DeFrancesco

doi:10.1038/nbt1008-1100

Launching the world's first commercial human genome sequencing center is currently the 'unreasonable' career ambition of sequencing-by-hybridization pioneer Rade Drmanac.

 

新闻特写：

Fixing the front end
Ken Garber

doi:10.1038/nbt1008-1101

One bottleneck in next-generation sequencing is genomic sample selection. As research groups tackle the problem, companies are seizing a market opportunity. Ken Garber reports.


What price personal genome exploration?
Jeffrey L Fox

doi:10.1038/nbt1008-1105

Companies offering direct-to-consumer genomic information face tough questions about who regulates them, where they fit in health care and how to value their services. What will it take to move them from niche services to a broader customer base? Jeffrey Fox reports.



The sequencing shakeup
Amy Coombs

doi:10.1038/nbt1008-1109

Deep sequencing technology could soon be competitive with certain array applications. But the jury remains out on which of the myriad platforms will have the greatest impact and broadest application. Amy Coombs investigates.

 

评论：

How to get genomes at one ten-thousandth the cost
Jeffery A Schloss

doi:10.1038/nbt1008-1113

The NHGRI's Advanced DNA Sequencing Technology program is spearheading the development of platforms that will bring routine whole-genome sequencing closer to reality.

 

研究成果：

The development and impact of 454 sequencing - pp1117 - 1124
Jonathan M Rothberg & John H Leamon

doi:10.1038/nbt1485


What would you do if you could sequence everything? - pp1125 - 1133
Avak Kahvejian, John Quackenbush & John F Thompson

doi:10.1038/nbt1494

（生物通：张迪）