北京生科所周俭民小组国际杂志发表新文章

　　

生物通综合：北京生命科学研究所的消息，2007年5月16日，我所周俭民研究小组在《Cell  Host  &  Microbe》上发表了题为  “A  Pseudomonas  syringae  Effector  Inactivates  MAPKs  to  Suppress  PAMP-Induced  Immunity  in  Plants”  的论文，报道了他们关于假单胞杆菌效应蛋白致病机理的新发现。

 

假单胞杆菌三型分泌系统分泌的效应蛋白是毒性假单胞杆菌最重要的致病武器，但是目前对这些效应蛋白的生化功能和它们在宿主体内的直接作用位点还知之甚少。该论文报道假单胞杆菌Pseudomonas  syringae  pv.  tomato  DC3000的效应蛋白HopAI1能够广泛抑制植物先天免疫反应，并证明拟南芥丝裂原活化蛋白激酶（MAPK,  Mitogen-activated  protein  kinase）MPK3和MPK6是HopAI1在植物体内的直接毒性靶位点。HopAI1属于一个在植物和动物病原菌中广泛存在并且保守的效应蛋白家族，HopAI1能直接与拟南芥MPK3和MPK6相互作用，并通过独特的磷酸化苏氨酸裂解酶活性使其去磷酸化并失活，揭示了细菌抑制宿主防卫反应的一个新的作用机制。同时该研究还分析了MAPK下游信号途径，表明MAPK通过激活与活性氧调节细胞壁防卫反应。

 

博士生张杰为本论文第一作者，论文的其他作者还有邵峰博士、柴继杰博士、李燕、崔海涛、陈琳洁、李洪涛博士、邹燕、蛋白质中心的龙承租和陈涉，以及堪萨斯州立大学的兰乐夫博士和唐晓艳博士。周俭民博士为本文通讯作者。该项目由科技部863项目和北京市政府资助。

 

周俭民 博士

 

教育经历

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1984	四川大学生物系学士
1994	普渡大学园艺学博士

工作经历

 

2004-present	中国北京生命科学研究所高级研究员
2002-2005	堪萨斯州立大学植物病理系副教授
1997-2002	堪萨斯州立大学植物病理系助理教授
1994-1997	普渡大学博士后研究助理
1989-1994	普渡大学博士生研究助理
1987-1989	中国科学院遗传研究所研究助理
1984-1987	中国科学院遗传研究所硕士生研究助理

研究概述：

本实验室的主要研究兴趣是植物与病原微生物间相互作用的机理。植物可以感受病原菌的入侵，并且启动防卫反应成功地抵御病原菌的感染。而病原菌通过适应寄主产生相应的毒性机制感染寄主，引发病害。我们用拟南芥和假单孢杆菌间互作为模式回答植物的抗病机理和病原菌的致病机理。目前的工作主要回答植物是如何识别细菌并激活先天免疫的信号传导通路，细菌致病蛋白的生化功能及在寄主中的靶点等问题。对这些问题的回答，有利于农业生产中对病害的控制。另外，因为动植物先天免疫机理的相似性和植物遗传易操作性，我们的工作还有助对动物先天免疫的认识。

Publications:

1.	Jie Zhang, Feng Shao, Yan Li, Haitao Cui, Linjie Chen, Hongtao Li, Yan Zou, Lefu Lan, Jijie Chai, Xiaoyan Tang, and Jian-Min Zhou (2007). A Pseudomonas syringae Effector Inactivates MAPKs to Suppress PAMP-Induced Immunity in Plants. Cell Host and Microbe In press.
2.	Yulei Shang, Xinyan Li, Haitao Cui, Ping He, Roger Thilmony, Satya Chintamanani, Julie Zwiesler-Vollick, Suresh Gopalan, Xiaoyan Tang, and Jian-Min Zhou (2006). RAR1, a central player in plant immunity, is targeted by Pseudomonas syringae effector AvrB. PNAS (track II), 103: 19200-19205.
3.	Li X., Lin H., Zhang W., Zou Y., Zhang J., Tang X., and Zhou J.M.. Flagellin induces innate immunity in nonhost interactions that is suppressed by Pseudomonas syringae effectors. PNAS (track II), 2005；102, 12990-12995.
4.	Xiao F., S. Goodwin M., Xiao Y., Sun Z., Baker D., Tang X., Jenks M. A., and Zhou J.M.. Arabidopsis CYP86A2 represses Pseudomonas syringae type III genes and is required for cuticle development. EMBO J.. 2004; 23, 2903-2913.
5.	Thara K. Venkatappa, Alexander R. Seilaniantz, Youping Deng, Yinghua Dong, Yinong Yang, Xiaoyan Tang, and Jian-Min Zhou (2004) Tobacco Genes Induced by the Bacterial Effector Protein AvrPto. MPMI, 1139-1145.
6.	He P., Chintamanani S., Chen Z., Zhu L., Kunkel B. N., Alfano J. R., Tang X., and Zhou J.M.. Activation of a COI1-dependent pathway in Arabidopsis by Pseudomonas syringae type III effectors and coronatine. Plant J., 2004; 37, 589-602.
7.	Kang L., Li J., Zhao T., Xiao F., Tang X., Thilmony R., He S.Y., and Zhou J.M.. Interplay of the Arabidopsis nonhost resistance gene NHO1 with bacterial virulence. PNAS (track II), 2003; 100, 3519-3524.
8.	Jian-Min Zhou and Xiaoyan Tang (2002). Plant disease resistance genes in gene-for-gene resistance and general resistance. In: Andrew J. Wood ed. Biochemical and Molecular Responses of Plants to the Environment, Biochemical & Molecular Responses of Plants to the Environment. Research Signpost, Trivandrum India. pp127. ISBN: 81-7736-167-8.
9.	He P., Friebe B., Gill B., and Zhou J.M.. Allopolyploidy alters gene expression in the highly stable hexaploid wheat. Plant Mol. Biol., 2003; 52, 401-414.
10.	Thara Venkatappa, John Fellers, and Jian-Min Zhou (2003). In planta induced genes of Puccinia triticina. Molecular Plant Pathology, 4, 51-56.
11.	Xiao F., Lu M., Zhao T., Tang X., and Zhou J.M.. Pto mutants differentially activate Prf-dependent, AvrPto-independent resistance and gene-for-gene resistance. Plant Physiol. 2003; 131, 1239-1249.
12.	Jianxiong Li, Libo Shan, Jian-Min Zhou, and Xiaoyan Tang (2002). Overexpression of Pto induces a salicylate-independent cell death but inhibits necrotic lesions caused by salicylate deficiency in tomato plants. MPMI, 15, 654-661.
13.	Xiao F.M., Tang X., and Zhou J.M.. Expression of 35S::Pto globally activates defense gene expression in tomato plants. Plant Physiol. 2001; 126, 1637-1645.
14.	Ping He, Randall F. Warren, Libo Shan, Tiehan Zhao, Lihuang Zhu, Xiaoyan Tang, and Jian-Min Zhou (2001). Overexpression of Pti5 potentiates pathogen-induced defense gene expression in tomato. MPMI 14, 1453-1457.
15.	Lu M., Tang X., and Zhou J.M.. Arabidopsis NHO1 is required for general resistance against Pseudomonas bacteria. Plant Cell. 2001; 13, 437-447.
16.	Libo Shan, Venkatappa Thara, Gregory Martin, Jian-Min Zhou, and Xiaoyan Tang (2000). The Pseudomonas AvrPto protein is differentially recognized by tomato and tobacco and is localized to the plant plasma membrane. Plant Cell, 12, 2323-2338.
17.	Libo Shan, Ping He, Jian-Min Zhou, and Xiaoyan Tang (2000). A cluster of mutations disrupt the avirulence but not the virulence function of AvrPto. MPMI 13, 592-598.
18.	Yong-Qiang Gu, Caimei Yang, Venkatappa Thara, Jian-Min Zhou, and Gregory Martin (2000). Pti4 is induced by ethylene and salicylic acid, and its product is phosphorylated by the Pto kinase. Plant Cell 12, 771-785.
19.	Venkatappa K. Thara, Xiaoyan Tang, Gregory B. Martin, and Jian-Min Zhou (1999). Pseudomonas syringae pv tomato induces the expression of tomato EREBP-like genes Pti4 and Pti5 independent of ethylene, salicylate and jasmonate. Plant J. 20, 475-483.
20.	Jian-Min Zhou (1999). Signal transduction and pathogen-induced PR gene expression. In: Pathogenesis-related proteins in plants, S.K. Datta and S. Muthukrishnan eds., CRC Press. 195-206.
21.	Xiaoyan Tang, Mingtang Xie, Young Jin-Kim, Jian-Min Zhou, Daniel F. Klessig, and Gregory B. Martin (1999). Overexpression of Pto Activates Defense Responses and Confers Broad Resistance. Plant Cell 11, 15-30.
22.	Ying-Tsu Loh, Jian-Min Zhou, and Gregory Martin (1998). The myristylation motif of Pto is not required for disease resistance. Mol. Plant-Microbe Interct. 11, 572-576.
23.	Ping Xu, Meena Narasimhan, Teresa Samson, Maria Coca, Gyung-Hye Huh, Jian-Min Zhou, Gregory Martin, Paul Hasegawa, and Ray Bressan (1998). A nitrilase-like protein interacts with GCC box DNA-binding proteins involved in ethylene and defense responses. Plant Physiology 118, 867-874.
24.	Jian-Min Zhou, Xiaoyan Tang, Reid Frederick, and Gregory Martin (1998). Pathogen recognition and signal transduction by the Pto kinase. J. Plant Res. 111, 353-356.
25.	Jian-Min Zhou, Xiaoyan Tang, and Gregory Martin (1997). The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes. EMBO Journal 16, 3207-3218.
26.	Yulin Jia, Ying-Tsu Loh, Jian-Min Zhou, and Gregory Martin (1997). Alleles of Pto and Fen occur in bacterial speck-susceptible and fenthion-insensitive tomato lines and encode functional protein kinases. Plant Cell 9, 61-73.
27.	Angus Murphy, Jian-Min Zhou, Peter B. Goldsbrough, and Lincoln Taiz (1997). Purification and immunological identification of metallothioneins 1 and 2 from Arabidopsis thaliana. Plant Physiology 113, 1293-1301.
28.	Jianjun Chen, Jian-Min Zhou, and Peter B. Goldsbrough (1997). Characterization of phytochelatin synthase from tomato. Physiologia Plantarum 101, 165-172.
29.	Martin, G.B., Xiaoyan Tang, Jianmin Zhou, Reid Frederick, Yulin Jia, and Ying-Tsu Loh (1996). Signal recognition and transduction in bacterial speck disease resistance of tomato. In: Biology of Plant-Microbe Interactions, G. Stacey et al. eds. (International Society for Molecular-Microbe Interactions, St. Paul, Minnesota).
30.	Martin, G.B., Reid Frederick, Roger L. Thilmony, and Jianmin Zhou (1996). Signal transduction events involved in plant disease resistance. In: Molecular Aspects of Pathogenicity and Resistance: Requirement for Signal Transduction, D. Mills, H. Kunoh, N. T. Keen and S. Mayama eds. U.S./Japan Seminar Series Proceedings, pp. 163-176.
31.	Xiaoyan Tang, Reid D. Frederick, Jianmin Zhou, Dennis A. Halterman, Yulin Jia, and Gregory B. Martin (1996). Physical interaction of AvrPto and the Pto kinase defines a recognition event involved in plant disease resistance. Science 274, 2060-2063.
32.	Jianmin Zhou, Ying-Tsu Loh, Ray Bressan, and Gregory Martin (1995). The tomato gene Pti1 encodes a serine/threonine kinase that is phosphorylated by Pto and is involved in the hypersensitive response. Cell 83, 925-935.
33.	Jianmin Zhou and Peter B. Goldsbrough (1995). Structure, organization and expression of Arabidopsis metallothionein gene family. Mol. Gen. Genet. 248, 318-328.
34.	Jianmin Zhou and Peter B. Goldsbrough (1994). Functional homologues of animal and fungal metallothionein genes from Arabidopsis. Plant Cell 6, 875-884.
35.	Jianmin Zhou and Peter B. Goldsbrough (1993). An Arabidopsis gene with homology to glutathione s-transferases is regulated by ethylene. Plant Molecular Biology 22, 517-523.
36.	Jiaping Zhou, Jianmin Zhou, Sixin Liang, He Huang, Long Pang, and Yinxian Jiao (1990). Cell selection of the tobacco mutant resistant to black shank disease. Acta Genetica Sinica 17(3), 180-188.

 

“万古霉素产业化关键技术”在沪获奖

 

昆明植物所刘吉开课题组项目通过成果鉴定

 

哈佛通过30年来首次课程大调整计划