北京生科院973项目最新文章解析修复机制

　　

生物通综合：来自北京生命科学研究所，中国农业大学生科院，山东聊城大学（Liaocheng University），以及美国亚利桑那州大学植物科学系的研究人员在拟南芥研究中发现一种突变体：SAD2（sensitive to ABA and drought2），为研究植物抵抗紫外线机制，以及为改善强紫外线对作物造成的危害状况提供一定的理论基础。

文章的通讯作者是来自北京生命科学研究所的郭岩博士，博士生赵金凤为本文第一作者。论文的其他作者还有赵杨，龚喜明等。郭岩博士为本文通讯作者。此项研究由科技部863，973计划，北京市科委资助，在北京生命科学研究所完成。

大气中的臭氧层能够屏蔽宇宙射线中的全部UV-C和大部分的UV-B到达地球表面，防止它们对生物体产生伤害。伴随人类对氯氟碳chlorofluorocarbons (CFCs)，氯碳化合物chlorocarbons (CCs)使用量的增加，大气层中的臭氧层受到日益严重的破坏。植物如何应答UV-B引起了更多的关注。目前为止已知植物应答UV-B主要通过两个分子机制：其一，植物主要通过合成紫外吸收物质，如flavonoids，sinapate esters等。这些物质积累在植物细胞中防止UV-B对植物体内部的伤害。其二，主要是对UV-B引起的植物体损伤进行修复。

我所郭岩实验室分离到一个突变体sad2，SAD2编码一个类核运输受体（importin β-like protein），主要功能是负责把胞质中的一些蛋白质运到细胞核中。他们的研究发现，sad2能够抵抗紫外线的伤害主要是由于在sad2体内组成性的积累了较多的flavonoids，sinapate esters，而在修复机制上野生型和突变体没有明显差别。SAD2能够负责把AtMYB4运到细胞核中，AtMYB4是紫外吸收物质sinapate esters合成的负调控因子。他们同时发现AtMYB4自身的mRNA和蛋白之间存在一个负反馈调节环。

通过对UV-B具有一定抗性的sad2突变体的研究分析，研究者希望为改善强紫外线对作物造成的危害状况提供一定的理论基础。

原文摘要：
Plant Cell Preview
Published on November 9, 2007; 10.1105/tpc.106.048900
SAD2, an Importin -Like Protein, Is Required for UV-B Response in Arabidopsis by Mediating MYB4 Nuclear Trafficking
『Abstract』

附：
郭岩 博士
北京生命科学研究所研究员
E-mail：[e-mail]


教育经历

1988
北京农业大学农学系植物育种学专业学士

1999
德国科隆大学遗传学系、德国科隆马普植物育种所植物分子遗传学博士


工作经历

2004-present
中国北京生命科学研究所工作


1999-2003
美国亚利桑那州图森市亚利桑那大学植物科学系朱健康博士实验室做博士后研究

1995-1999
德国科隆马普育种所Richard D.Thompson 博士实验室读博士

1988-1999
中国北京中国科学院遗传所陈受宜教授实验室做研究助理


研究概述：

本实验室的研究兴趣主要是植物如何感受并响应环境胁迫，诸如土壤高浓度盐、碱，及干旱，特别是植物在胁迫条件下体内Ca2+ 信号和pH内平衡的调节。主要研究内容是利用拟南芥突变体研究植物感受环境胁迫信号过程中所发生的遗传和分子特性的变化。目的是为了阐明植物响应环境胁迫的信号路径，并鉴定一些具有潜在功能的能够提高作物适应环境胁迫的关键作用元件。

Publications:

1.
Quan, R., Lin, H., Mendoza, I., Zhang, Y., Cao, W., Yang, Y., Shang, M., Chen, S., Pardo, J. M., and Guo, Y. 2007. SCaBP8/CBL10, a Putative Calcium Sensor, Interacts with the Protein Kinase SOS2 to Protect Arabidopsis Shoots from Salt Stress. The Plant Cell. Published online April 20, 2007.

2.
Guo, Y., Qiu, Q.-S., Schumaker, K.S., and Zhu, J.-K. 2004. Transgenic evaluation of activated mutant alleles of SOS2 reveals a critical requirement of its kinase activity and C-terminal regulatory domain for salt tolerance in Arabidopsis. Plant Cell 16: 435-449.

3.
Gong D., Guo Y., Schumaker K. S., and Zhu J-K. 2004. The SOS3 Family of Calcium Sensors and SOS2 Family of Protein Kinases in Arabidopsis. Plant Physiol. 134: 919-926.

4.
Qiu, Q.-S., Guo, Y., Quintero F.j., Pardo J.M., Schumaker, K.S. and Zhu, J.-K. 2004. Regulation of vacuolar membrane Na+/H+ exchange in Arabidopsis thaliana by the SOS pathway. J Biol Chem. 279(1): 207-215.

5.
Ohta, M., Guo, Y., Halfter, U. and Zhu J.K. 2003. A novel domain in the protein kinase SOS2 mediates interaction with the protein phosphatase 2C ABI2. Proc. Natl. Acad. Sci. USA. 100:11771-11776.

6.
Shi, H., Kim, Y.-S., Guo Y., and Zhu, J.-K. 2003. The Arabidopsis SOS5 Locus Encodes a Putative Cell Surface Adhesion Protein and Is Required for Normal Cell Expansion. Plant Cell 15: 19-32.

7.
Guo, Y., Xiong, L., Ishitani, M. and Zhu, J.-K. 2002. An Arabidopsis mutation in translation elongation factor 2 causes superinduction of CBF/DREB1 transcription factor genes but blocks the induction of their downstream targets under low temperatures. Proc. Natl. Acad. Sci. USA. 99:7786-7791.

8.
Guo, Y., Xiong, L., Song, C.-P., Gong, D., Halfter, U. and Zhu, J.-K. 2002. A calcium sensor and its interacting protein kinase are global regulators of abscisic acid signaling in Arabidopsis. Dev. Cell 3:233-244.

9.
Qiu, Q.-S., Guo, Y., Dietrich, M.A., Schumaker, K.S. and Zhu, J.-K. 2002. Regulation of SOS1, a plasma membrane Na+/H+ exchanger in Arabidopsis thaliana, by SOS2 and SOS3. Proc. Natl. Acad. Sci. USA. 99:8436-8441.

10.
Gong, D., Guo, Y., Jagendorf, A.T. and Zhu, J.-K. 2002. Biochemical characterization of the Arabidopsis protein kinase SOS2 that functions in salt tolerance. Plant Physiol. 130:256-264.

11.
Santandrea, G., Guo, Y., O'Connell, T. and Thompson, R.D. 2002. Post-phloem protein trafficking in the maize caryopsis: ZmTRXh1, a thioredoxin specifically expressed in the pedicel parenchyma of Zea mays L., is found predominantly in the placentochalaza. Plant Mol. Biol. 50:743-756.

12.
Lee, H., Guo, Y., Ohta, M., Xiong, L., Stevenson, B. and Zhu, J.-K. 2002. LOS2, a genetic locus required for cold-responsive gene transcription encodes a bi-functional enolase. EMBO J. 21:2692-2702.

13.
Gong, D., Gong, Z., Guo, Y., Chen, X. and Zhu, J.-K. 2002. Biochemical and functional characterization of PKS11, a novel Arabidopsis protein kinase. J. Biol. Chem. 277:28340-28350.

14.
Gong, D., Gong, Z., Guo Y. and Zhu. J.-K. 2002. Expression, activation, and biochemical properties of a novel Arabidopsis protein kinase. Plant Physiol. 129:225-234.

15.
Gómez, E., Royo, J., Guo, Y., Thompson, R. and Hueros, G. 2002. Establishment of cereal endosperm expression domains: Identification and properties of a maize transfer cell–specific transcription factor, ZmMRP-1. Plant Cell 14:599-610.

16.
Guo, Y., Halfter, U., Ishitani, M. and Zhu, J.-K. 2001. Molecular characterization of functional domains in the protein kinase SOS2 that is required for plant salt tolerance. Plant Cell 13:1383-400.

17.
Xiong, L., Gong, Z., Rock, C.D., Subramanian, S., Guo, Y., Xu, W, Galbraith, D. and Zhu, J.-K. 2001. Modulation of abscisic acid signal transduction and biosynthesis by an Sm-like protein in Arabidopsis. Dev Cell. 1:771-781.

18.
Guo, Y., Zhang, L., Xiao, G., Cao, S., Gu, D., Tian, W. and Chen, S. 1997. Expression of the BADH gene and salinity tolerance in rice transgenic plants. Sci. in China 40:296-501.

19.
Guo, Y., Chen, S., Zhang, G. and Chen, S. 1997. Salt-tolerance in rice mutant lines controlled by a major effector gene was obtained by a cell engineering technique. Acta Genet. Sin. 24:122-126.

20.
Zhang,G.-Y., Guo, Y., Chen, S.-L. and Chen, S.-Y. 1995. RFLP tagging of a salt tolerance gene in rice. Plant Sci. 110:227-234.

21.
Guo, Y., Chen, S.-L., Zhang, G.-Y. and Chen, S. 1993. Study on selection of HYP-resistant variants of rice and their characteristics. Acta Biol. Exp. Sin. 126: 5-10.