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还沉浸于传统的基因克隆方法无法自拔吗?

        “从一开始我采用的就是传统的基因克隆方法,而且每个人
都在用。因此做克隆总是会想到连接酶!”

艾克隆
2011届硕士研究生

是时候改变自己的习惯了!


In-Fusion HD Cloning
Plus Systems


PCR克隆试剂盒——采用In-Fusion HD Cloning Plus进行高效DNA无缝克隆

       In-Fusion HD Cloning Plus是一款简便高效的克隆试剂盒,能够将单个或者多个DNA片段快速定向克隆到任意载体中。
Learn more now
  • 忘掉连接酶吧:不再需要连接酶 ,轻松实现基因克隆
  • 忘掉内切酶吧:酶切位点随便选,构建载体零阻力
  • 忘掉小捣蛋吧:避免冗余碱基,创建无缝重组载体
  • 忘掉亚克隆吧:多片段克隆,一步到位
多片段克隆
一步到位,无需亚克隆



点突变
缺失、添加或者替换——一个试剂盒全部搞定

操作流程(3步):

  1. 预设最终的重构体(任何载体)。
  2. 设计包含突变位点的引物并按照In-Fusion® HD Cloning Plus 操作手册进行实验
  3. 次日,获得突变体的效率可高达95%!

高通量克隆
        Oxford Protein Production Facility(OPPF)已采用Clontech In-Fusion 克隆系统构建了pOPIN vector 重组载体库 。OPPF科学家以703个PCR片段为目的片段成功构建了661重组载体,总体克隆效率可达到94%。文章已发表在Nucleic Acids Research上。

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“I’m In-fusion, I forgot cloning”

 
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由宝日医生物技术(北京)有限公司委托 生物通 设计制作