联系电话：0531-85912162
0531-88807138
0531-88807138
[ 查看部分文献引用 ]
细胞系 |
转染效率 (% GFP) |
细胞系 |
转染效率 (% GFP) |
McArdle 7777 |
65-70% |
SAOS-2 |
58% |
实例说明PolyJet™体外DNA转染试剂对常见细胞系优异的转染效率。
转染试剂操作步骤及其使用指南
- 一般性哺乳动物细胞转染操作步骤 
- 简易转染操作步骤(适合熟练操作者) .gif){kind=small}
- 悬浮293和CHO细胞转染操作步骤
- 对难转哺乳动物细胞转染操作步骤
- 包装慢病毒转染操作步骤
- 包装重组腺相关病毒转染操作步骤
- 注意事项及转染窍门
对PolyJet™体外DNA转染试剂感兴趣？网上注册即可收到一份免费试用样品。上网注册请点击账户注册，输入您的邮寄地址后，请发送邮件至info@signagen-china.com
用户评价：
PolyJet Transfection Reagent worked equally as well as lipofectamine 2000, with
little evidence of cell death on 293, PC-3 and 22RV1 cells. I will defiantly
consider switching over.
-----Tiffany Wallace, Ph.D., June 9, 2009, NCI / NIH
I only did side
by side with the testing sample (PolyJet) and Lipo2000 with GFP transfection on
COS-7 cells. The result was very good. PolyJet was even better than L2K.
------Feng Qiao, Ph.D., Jun 12, 2009, NEI / NIH
I tested the sample of
PolyJet on my NIH-3T3 mouse fibroblasts this weekend. The results were much
better than Lipofecatmine LTX. I'm attaching a powerpoint slide with my results
(I did not quantify the % transfection efficiency, but the pictures get the
point across). I found that the protocol for difficult-to-transfect cell lines
worked better than the standard protocol.
-----Stephanie Murphy, Ph.D., June 29, 2009, Dartmouth College
I had chance to try your product finally. It was great success. I used HeLa
cells and got 10% transfection efficiency (<0.1% for Lipofectamine). Thank you!
I was wondering if I also try GenJet™ Plus DNA In Vitro Transfection Reagent?
According to your website, the reagent works better than regular PolyJet.
-------Yumi Uetake, Ph.D., August11, 2009, UMASS
I tested PolyJet and it looks great on MDCK. We placed order. Thank you!
-------Ge Zhou, Ph.D., August 12, 2009, NYU
We are
happy to provide feedback. PolyJet worked very well for us in HepG2 cells, we
got approximately 80% efficiency with pMAX GFP plasmid, by following the
conditions in your suggested protocol. We ran a comparison with Lipofectamine,
which only showed approximately 20-30% transfection efficiency. We are
planning experiments and will be ordering more soon.
-------Emily
Mcallister, September 3rd, 2009, PBRC
美国SignaGen官方网站：http://signagen.com/ |