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| 研究发现，外泌体参与调控重要的细胞生理活动，如免疫应答、凋亡、血管生成、炎症反应等，其有望用于开发疾病治疗的潜在靶点、早期诊断标记物、靶向药物载体等，已成为近年来生命科学及医学领域的重要研究热点。 外泌体研究策略  注： miRNA、piRNA、lncRNA、mRNA等功能验证方案及涉及产品请咨询锐博生物技术人员 1、外泌体抽提【自主专利+客户文章1600+篇】 外泌体功能研究的关键前提是提取出高质量的外泌体，传统提取方法利用超速离心或密度梯度离心对外泌体进行富集，这些方法耗时耗力，提取效果或产量却并不理想。 锐博生物拥有自主专利的新型外泌体提取试剂盒，仅需通过简单混匀和常规离心，即可从样本中获取大量结构完整的外泌体，为您提供更便捷、高效、高纯度的提取方法。 【一种分离外泌体的方法及其试剂盒 专利申请号：CA201510469340.3】 产品特点：
代表性客户文章： [1] Sun L, Zhu W, Zhao P, et al. Long noncoding RNA UCA1 from hypoxia-conditioned hMSC-derived exosomes: a novel molecular target for cardioprotection through miR-873-5p/XIAP axis[J]. Cell death & disease, 2020, 11(8): 1-16.[2] Zhang K, Dong C, Chen M, et al. Extracellular vesicle-mediated delivery of miR-101 inhibits lung metastasis in osteosarcoma[J]. Theranostics, 2020, 10(1): 411. [3] Bai M, Li J, Yang H, et al. MiR-135b delivered by gastric tumor exosomes inhibits FOXO1 expression in endothelial cells and promotes angiogenesis[J]. Molecular Therapy, 2019. [4] Xu W, Wu Y, Hu Z, et al. Exosomes from Microglia Attenuate Photoreceptor Injury and Neovascularization in an Animal Model of Retinopathy of Prematurity[J]. Molecular Therapy-Nucleic Acids, 2019, 16: 778-790. [5] Nie X, Fan J, Li H, et al. miR-217 promotes cardiac hypertrophy and dysfunction by targeting PTEN[J]. Molecular Therapy-Nucleic Acids, 2018, 12: 254-266. 产品订购：
2、外泌体鉴定服务 a.粒径检测 Zetasizer 仪器根据Stokes-Einstein 原理，使用动态光散射检测由于颗粒布朗运动而产生散射光的波动随时间的变化。检测器将散射光信号转化为电流信号，再通过数字相关器的运算处理，得到颗粒在溶液中扩散的速度信息，即扩散系数。
Exosomes 常见的特异性标记蛋白为CD63 和CD81，使用荧光的直标抗体结合流式细胞仪能够统计出经分离的Exosomes 的阳性百分比，从而判断Exosomes 的纯度。
代表性客户文章： [1] Wang W, Li D B, Li R Y, et al. Diagnosis of hyperacute and acute ischaemic stroke: the potential utility of exosomal MicroRNA-21-5p and MicroRNA-30a-5p[J]. Cerebrovascular Diseases, 2018, 45(5-6): 204-212.[2] Chen J, Yu Y, Li S, et al. Micro RNA‐30a ameliorates hepatic fibrosis by inhibiting Beclin1‐mediated autophagy[J]. Journal of cellular and molecular medicine, 2017, 21(12): 3679-3692. 委托科研服务：
3、 外泌体RNA测序服务【完成＞1500例外泌体RNA建库，成功率＞95%】 a. 外泌体Small RNA测序
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代表性客户文章： [1] Jiang F, Chen Q, Wang W, et al. Hepatocyte-Derived extracellular vesicles promote endothelial inflammation and atherogenesis via microRNA-1[J]. Journal of Hepatology, 2020, 72(1): 156-166.[2] Shang A, Gu C, Wang W, et al. Exosomal circPACRGL promotes progression of colorectal cancer via the miR-142-3p/miR-506-3p-TGF-β1 axis[J]. Molecular cancer, 2020, 19(1): 1-15. [3] Huang W, Yan Y, Liu Y, et al. Exosomes with low miR-34c-3p expression promote invasion and migration of non-small cell lung cancer by upregulating integrin α2β1[J]. Signal Transduction and Targeted Therapy, 2020, 5(1): 1-13. [4] Fang S B, Zhang H Y, Wang C, et al. Small extracellular vesicles derived from human mesenchymal stromal cells prevent group 2 innate lymphoid cell-dominant allergic airway inflammation through delivery of miR-146a-5p[J]. Journal of extracellular vesicles, 2020, 9(1): 1723260. [5] Hou Z, Qin X, Hu Y, et al. Longterm exercise-derived exosomal miR-342-5p: a novel exerkine for cardioprotection[J]. Circulation research, 2019, 124(9): 1386-1400. 委托科研服务：
4、qPCR验证【自主专利产品】 miRNA qRT-PCR试剂盒 锐博生物提供拥有自主专利的茎环法和加尾法miRNA qPCR检测试剂盒。【高灵敏度小RNA定量检测方法及试剂盒，专利授权号：CA201410039162.6】 产品特点：
b. lncRNA qRT-PCR试剂盒 riboSCRIPT qRT-PCR Starter Kit 是锐博生物推出的基于SYBR Green I 嵌合荧光法的qRT-PCR 试剂盒，可实现lncRNA 或circRNA或mRNA 表达情况的快速、高效、准确的检测。 产品特点：
代表性客户文章： [1] Sun Y, Wang Q, Zhang Y, et al. Multigenerational maternal obesity increases the incidence of HCC in offspring via miR-27a-3p[J]. Journal of Hepatology, 2020, 73(3): 603-615.[2] Zhao Y, Zhao M F, Jiang S, et al. Liver governs adipose remodelling via extracellular vesicles in response to lipid overload[J]. Nature communications, 2020, 11(1): 1-17. [3] Ma H, Chang H, Yang W, et al. A novel IFNα-induced long noncoding RNA negatively regulates immunosuppression by interrupting H3K27 acetylation in head and neck squamous cell carcinoma[J]. Molecular cancer, 2020, 19(1): 1-16. [4] Qiao J, Zhao J, Chang S, et al. MicroRNA-153 improves the neurogenesis of neural stem cells and enhances the cognitive ability of aged mice through the notch signaling pathway[J]. Cell Death & Differentiation, 2020, 27(2): 808-825. [5] Wu P, Cai J, Chen Q, et al. Lnc-TALC promotes O 6-methylguanine-DNA methyltransferase expression via regulating the c-Met pathway by competitively binding with miR-20b-3p[J]. Nature communications, 2019, 10(1): 2045. 产品订购：
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