Total RNA was purified from human breast, liver, kidney FFPE samples using the RNeasy FFPE Kit. Duplex, real-time RT-PCR was performed with (+RT) or without (-RT) the reverse transcription step for a range of different target with different expression levels using QuantiFast Probe Assays (FAM labeled) and A the QuantiFast Probe RT-PCR Plus Kit or B an alternative kit for multiplex PCR which does not include an integrated gDNA removal step. All reactions were performed on the ABI StepOnePlus cycler. A High CT values in the –RT samples indicate no amplification and the absence of genomic DNA contamination. Therefore reliable and accurate detection of RNA. Target 12 (TUSC2) and 13 (EZH2) are not expressed. These results show that the QuantiFast Probe RT-PCR Plus Kit efficiently removes genomics DNA. B Similar CT values in both the –RT and +RT sample indicate that contaminating genomic DNA is being detected, making these results unreliable.