Attractene Transfection Reagents
对各种细胞进行高效质粒DNA转染，且极低毒性
■ 转染步骤快速、简单，无须提前一天铺细胞
■ 加入转染复合物后无须更换细胞培养液
■ 每孔用量*仅为常用脂质体的一半，价格更经济
Attractene 试剂采用非脂质体的脂质技术，经广泛测试，其
细胞毒性极低，对于多种细胞包括难转染的如原代细胞的转染
效率明显高于常用脂质体试剂，适合质粒DNA转染、DNA共转
或DNA/siRNA共转。
分享成功转染经验 — 来自中国的客户
原代细胞和干细胞
各种细胞系
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与转染试剂L2K及FG 比较，Attractene具有效率更高、毒性极低的优点
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图 1. Attractene效率更高. DNA (pGFP which expresses green fluorescent protein) was transfected into the cell types indicated using Attractene Reagent. Transfection were also performed with A Reagent F or B Reagent L according to the manufacturer’ handbooks. Transfection efficiency was estimated by counting the number of fluorescent cells by FACS® analysis. Transfection efficiency is expressed relative to efficiency using Attractene Reagent which was set at 100%. |
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图2. Attractene 毒性极低。HepG2 cells were transfected with DNA (pGFP) using Attractene Transfection Reagent or Reagent L from another supplier according to the manufacturer's instructions. Two days after transfection, cells were examined by light microscopy. Cells transfected using Attractene Reagent were healthy and viable. In contrast, cells transfected using Reagent L displayed high levels of cell death.
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欢迎致电QIAGEN中国咨询更多成功转染细胞的经验。 |
