产品介绍
EntransterTM-R4000是英格恩生物（Engreen Biosystem）最新研发合成的针对siRNA、microRNA、mimic、inhibitor、mRNA和shRNA等RNA的转染试剂。EntransterTM-R4000不仅可以转染小RNA，而且针对mRNA等长链RNA优化。由于纳米技术的应用，EntransterTM-R4000能做到高达90%的转染效率和60-90%的沉默效率，对难转染细胞和原代细胞也非常适用。
适用范围
●小RNA转染。包括siRNA转染，miRNA，mimic，inhibitor等。
●长链的mRNA和shRNA转染，针对mRNA和长链的shRNA等RNA转染优化。
●可转染动物细胞、昆虫细胞等，也可以转染原代细胞、悬浮细胞和原代细胞等传统难转染细胞。
主要特点
●纳米聚合物，转染效果优于脂质体转染试剂
●高效转染，沉默效率高，可以达到90％以上的沉默效率
●低细胞毒性，有血清转染，无须反复更换培养基
●原代细胞、悬浮细胞和敏感细胞都能转
产品列表
| Products |
Cat. No. |
Size |
Price |
| EntransterTM-R RNA4000 Transfection Reagent |
4000-3 |
0.75ml |
1199 |
| EntransterTM-R RNA4000 Transfection Reagent |
4000-4 |
1.5ml |
1999 |
应用本产品发表的部分文献
1. Tumour Biol. 2016 Mar. MiR-30a regulates the proliferation, migration, and invasion of human osteosarcoma by targeting Runx2.（MiR-30a通过靶向作用于小家畜相关的转录因子2（Runx2）进而调节人骨肉瘤细胞的增值、迁移及侵袭）
2. PLoS One. 2015 Mar 31. MicroRNA-22 impairs anti-tumor ability of dendritic cells by targeting p38.（MicroRNA-22 通过靶向作用于p38蛋白削弱树突状细胞的抗肿瘤能力）
3.Mol Biosyst. 2015 Nov The involvement of aquaporin 1 in the hepatopulmonary syndrome rat serum-induced migration of pulmonary arterial smooth muscle cells via the p38-MAPK pathway.（肝肺综合征相关的研究）
4. J Immunol. 2015 Aug 1. MicroRNA-9 Regulates the Differentiation and Function of Myeloid-Derived Suppressor Cells via Targeting Runx1（MicroRNA-9 通过靶向作用于Runx1调节髓源性抑制细胞的分化和功能）
5.Immunol Res. 2015 Jun. Decreased expression of microRNA-125a-3p upregulates interleukin-23 receptor in patients with Hashimoto’s thyroiditis.（microRNA-125a-3p的低表达可以上调淋巴瘤性（样）甲状腺肿病人白细胞介素-23受体的水平）.
6.Int J Mol Med. 2015 Dec. miR-21 synergizes with BMP9 in osteogenic differentiation by activating the BMP9/Smad signaling pathway in murine multilineage cells.（miR-21激活小鼠干细胞中BMP9 /Smad信号通路与BMP9 协同作用于成骨细胞分化的研究）
7.J Biol Chem. 2010 Apr 2;285(14):10890-901.Xenopus skip modulates Wnt/beta-catenin signaling and functions in neural crest induction.
8.Apoptosis. 2010 Apr;15(4):488-98.A PKC-beta inhibitor protects against cardiac microvascular ischemia reperfusion injury in diabetic rats.
9.Microvasc Res. 2010 Jan 14. A PKC-beta inhibitor treatment reverses cardiac microvascular barrier dysfunction in diabetic rats.
10.J Immunol. 2011 Apr 15;186(8):4716-24.Both miR-17-5p and miR-20a Alleviate Suppressive Potential of Myeloid-Derived Suppressor Cells by Modulating STAT3 Expression.
11.J Sex Med. 2011 Oct;8(10):2773-80.Up-regulation of VEGF by small activator RNA in human corpus cavernosum smooth muscle cells.
12.Cell Biochem Funct. 2011 Oct 26.The effects of myocyte enhancer factor 2A gene on the proliferation, migration and phenotype of vascular smooth muscle cells.
13.Virus Res. 2012 Jan;163(1):183-9.Small interfering RNA against the 2C genomic region of coxsackievirus B3 exerts potential antiviral effects in permissive HeLa cells.
14.Genes Immun. 2012 Jan 26.Global mapping of H3K4me3 and H3K27me3 reveals chromatin state-based regulation of human monocyte-derived dendritic cells in different environments.
15.Journal of Radiation Research. 2012 June 6. MicroRNA-148b enhances the radiosensitivity of non-Hodgkin’s Lymphoma cells by promoting radiation-induced apoptosis.
16.J Cell Sci. 2012 May 28. Regulation of apoptosis by Bat3-enhanced YWK-II protein/APLP2 stability.
17.Cytokine. 2012 Jul 29. BLyS expression and JNK activation may form a feedback loop to promote survival and proliferation of multiple myeloma cells.
18.Genes Immun. 2012 Oct 4.TGF-β-associated miR-27a inhibits dendritic cell-mediated differentiation of Th1 and Th17 cells by TAB3, p38 MAPK, MAP2K4 and MAP2K7.
19.Respir Physiol Neurobiol. 2012 Oct 8.Effect of annexin A2 on hepatopulmonary syndrome rat serum-induced proliferation of pulmonary arterial smooth muscle cells.
20.Biochem J. 2012 Nov 1;447(3):407-16.Promoter-associated small double-stranded RNA interacts with heterogeneous nuclear ribonucleoprotein A2/B1 to induce transcriptional activation.
21.Cell Signal. 2013 Jan 15.SASH1 regulates melanocyte transepithelial migration through a novel Gαs-SASH1- IQGAP1-E-Cadherin dependent pathway.
22.J Immunol. 2013 Jan 25.Multiple Tumor-Associated MicroRNAs Modulate the Survival and Longevity of Dendritic Cells by Targeting YWHAZ and Bcl2 Signaling Pathways.
23.Apoptosis. 2013 May 25.The histone deacetylase inhibitor vorinostat prevents TNFα-induced necroptosis by regulating multiple signaling pathways.
24.Molecular BioSystems. 2013 Nov 29.Paxillin suppresses the proliferation of HPS rat serum treated PASMCs by up-regulating the expression of cytoskeletal proteins.
相关资料
● RNA转染技术资料下载
相关问题
● 为什么Entranster™ 转染试剂有血清转染不仅没有毒性，反而有助于提高转染效率?
● 成功siRNA转染的主要关键点有什么？
● siRNA转染与DNA转染有什么不一样?
● siRNA转染后什么时候做qRT-PCR最好？
● siRNA转染后什么时候做蛋白检测检测最好？
● siRNA转染细胞铺板密度为什么重要？
● 为什么脂质体转染试剂细胞毒性大?
● 脂质体毒性为什么可能影响实验结果？
● Entranster™ 系列转染试剂的成分和原理？
相关试剂
Entranster™ -D4000转染试剂（最新转染试剂）
Entranster™ -in vivo转染试剂（用于动物体内转染）
Entranster™ -H4000转染试剂（常规转染试剂）
存储和运输
常温运输，储存于4℃。
安全性
本品使用安全，未发现任何生物、化学毒性。如不慎沾染，用清水冲洗即可。
Material Safety Data Sheets (MSDS)
质量保证
北京英格恩生物科技有限公司对EntransterTM-R基因转染试剂的每批产品实行严格质量检验，并进行转染验证，以确保产品质量。请用户使用前务必认真阅读 操作手册。如用户仍然有问题，请立即联系本公司人员，英格恩公司将负责提供全程技术服务。
使用限制
本品仅限科研用途。
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