首款专注于tRNA研究的PCR芯片——nrStar™ Human tRNA Repertoire PCR Array

转运RNA (tRNA) 是生物体内分布最广泛、含量最丰富的非编码小RNA分子。它携带并转运氨基酸，参与蛋白翻译，是连接mRNA与蛋白质的重要桥梁。细胞增殖[1]、分化[1, 2]和凋亡[3]等一系列生物学过程都伴随着tRNA水平的变化。反之，tRNA表达谱的改变也会影响细胞发育过程中的命运抉择。表达失调的tRNA可以促进肿瘤的发生和癌症进程[2, 4-11]。另外，许多其它疾病比如II型糖尿病[12]、亨廷顿症[13]以及HIV感染[14]都出现了tRNA表达与分布紊乱。tRNA研究已逐渐成为生物学过程和疾病研究的重要组成部分。

Arraystar最新发布了市场上首款专注于tRNA研究的PCR芯片——nrStar™ Human tRNA Repertoire PCR Array。该款芯片可同时检测66个细胞核tRNA和22个线粒体tRNA，覆盖了tRNA权威数据库GtRNAdb和tRNAdb中的所有反密码子，方便客户快速地进行tRNA表达谱分析，为下一步的密码子偏好性、蛋白翻译效率和准确性、细胞动力学以及病毒感染的细胞嗜性等研究提供重要线索，是进行tRNA研究必不可少的工具。

tRNA存在种类繁多的修饰，对其发挥功能十分关键。然而，这些修饰尤其是甲基化修饰会严重阻碍反转录的进行，导致cDNA合成终止或碱基错配。为此，Arraystar专门开发了针对tRNA的反转录试剂盒(rtStar™ tRNA-optimized First-Strand Synthesis Kit)。该试剂盒采用了一种高效的RNA去甲基化酶AlkB，能够有效的去除tRNA上的甲基化修饰，极大的提高cDNA合成质量。与此试剂盒组合使用，研究人员能够获得更为真实可靠的tRNA表达变化，为研究蛋白质组或tRNA来源片段（tRFs）提供重要信息。

为了保证数据可信度，芯片还包含了3个看家小RNA作为内参，以及3个质控对照RNA Spike-in、PCR阳性对照（PPC）和基因组DNA对照（GDC）,分别用于监测cDNA合成质量、PCR效率和基因组DNA污染。

在后续研究中，敲低tRNA[15] 或过表达tRNA [2, 16, 17]可以进一步揭示特定tRNA在细胞生命活动中的作用。其它用于非编码RNA研究的相关技术也适用于tRNA的后续研究。

产品列表

产品名称	规格	描述
nrStar™ Human tRNA Repertoire PCR Array	384-well (4*96) plate	包括66个细胞核tRNA和22个线粒体tRNA

芯片特点

• 囊括GtRNAdb和tRNAdb数据库中所有的细胞核与线粒体反密码子
• 伴随的去甲基化处理使得检测结果更加真实可靠
• 所有引物均在多种细胞和组织中通过验证
• 即拆即用型384孔板，几小时内便可得到结果

工作流程

 
适用实时定量荧光PCR仪：

ABI ViiA™ 7，ABI 7500 & ABI 7500 FAST，ABI 7900HT，ABI QuantStudio™ 6 Flex Real-Time PCR system，ABI QuantStudio™ 7 Flex Real-Time PCR system，ABI QuantStudio™ 12K Flex Real-Time PCR System，Bio-Rad CFX384，Bio-Rad iCycler & iQ Real-Time PCR Systems，Eppendorf Realplex，QIAGEN Rotor Gene Q100，Roche LightCycler 480，Stratagene Mx3000，Roche LightCycler 480

数据库

tRNA反密码子信息(GtRNAdb和tRNAdb数据库)：

细胞核tRNA (66): Ala-AGC, Ala-CGC, Ala-GGC, Ala-TGC, Arg-ACG, Arg-CCG, Arg-CCT, Arg-TCG, Arg-TCT, Asn-ATT, Asn-GTT, Asp-ATC, Asp-GTC, Cys-GCA, Gln-CTG-1, Gln-CTG-2, Gln-TTG-1, Gln-TTG-2, Glu-CTC, Glu-TTC, Gly-CCC-1, Gly-CCC-2, Gly-GCC, Gly-TCC, His-GTG, Ile-AAT, Ile-TAT, Ini-CAT, Leu-AAG, Leu-CAA, Leu-CAG, Leu-TAA, Leu-TAG, Lys-CTT-1, Lys-CTT-2, Lys-TTT, Met-CAT, Phe-GAA, Pro-AGG, Pro-CGG, Pro-GGG, Pro-TGG, Sec-TCA, Ser-ACT, Ser-AGA, Ser-CGA, Ser-GCT, Ser-GGA, Ser-TGA, Sup-CTA, Sup-TTA, Thr-AGT-1, Thr-AGT-2, Thr-CGT, Thr-TGT-1, Thr-TGT-2, Trp-CCA, Tyr-ATA, Tyr-GAT, Tyr-GTA, Val-AAC, Val-CAC-1, Val-CAC-2, Val-CAC-3, Val-TAC-1, Val-TAC-2

线粒体tRNA (22): mt-Ala-TGC, mt-Arg-TCG, mt-Asn-GTT, mt-Asp-GTC, mt-Cys-GCA, mt-Gln-TTG, mt-Glu-TTC, mt-Gly-TCC, mt-His-GTG, mt-Ile-GAT, mt-Leu-TAA, mt-Leu-TAG, mt-Lys-TTT, mt-Met-CAT, mt-Phe-GAA, mt-Pro-TGG, mt-Ser-GCT, mt-Ser-TGA, mt-Thr-TGT, mt-Trp-TCA, mt-Tyr-GTA, mt-Val-TAC

立即索取nrStar Human tRNA Repertoire PCR Array的详细资料

参考文献
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