复旦讲座教授韩珉最新文章

　　

生物通报道：复旦大学讲座特聘教授韩珉、徐人尔教授带领的研究小组在最近的PNAS上发表新的研究进展，SUN1 and SUN2 play critical but partially redundant roles in anchoring nuclei in skeletal muscle cells in mice。

 

韩珉教授和徐人尔教授都是复旦大学发育生物学研究所的成员，韩珉教授从事发育生物学研究，徐人尔教授从事分子和西部生物学研究，早在2007年，韩珉教授就曾与徐人尔教授合作在《Developmental Cell》发表SUN1对生殖发育的研究成果。证实SUN1蛋白变异是生殖细胞形成异常的关键因素。

 

“SUN1”蛋白能够帮助父母双亲来源的染色体结合到核膜上启动染色体配对(遗传信息减半传递的第一步)和遗传重组。而根据通过对小鼠的实验，如果“SＵN1”基因突变，就会使染色体配对和遗传重组受阻而不能产生成熟的精子和卵子，造成突变小鼠不育。

 

本次，两人再度联手在PNAS上发表文章，本次讲述的是SUN1和SUN2在小鼠的骨骼肌发育过程中的关键作用，两种蛋白主要影响细胞核的定位，主要通过Syne-1的定位影响核膜的定位。

（生物通 小茜）

生物通推荐原文检索：

 

SUN1 and SUN2 play critical but partially redundant roles in anchoring nuclei in skeletal muscle cells in mice

 

Kai Leia,1, Xiaochang Zhanga,1, Xu Dinga, Xue Guoa, Muyun Chena, Binggen Zhua,2, Tian Xua,b, Yuan Zhuanga,c, Rener Xua,3 and Min Hana,d,3

-Author Affiliations

 

aInstitute of Developmental Biology and Molecular Medicine, School of Life Science, Fudan University, Shanghai 200433, China;

bHoward Hughes Medical Institute and Department of Genetics, Yale University School of Medicine, New Haven, CT 06520;

cDepartment of Immunology, Duke University Medical Center, Durham, NC 27710; and

dHoward Hughes Medical Institute and Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309

 

【Abstract】

How the nuclei in mammalian skeletal muscle fibers properly position themselves relative to the cell body is an interesting and important cell biology question. In the syncytial skeletal muscle cells, more than 100 nuclei are evenly distributed at the periphery of each cell, with 3–8 nuclei anchored beneath the neuromuscular junction (NMJ). Our previous studies revealed that the KASH domain–containing Syne-1/Nesprin-1 protein plays an essential role in anchoring both synaptic and nonsynaptic myonuclei in mice. SUN domain–containing proteins (SUN proteins) have been shown to interact with KASH domain–containing proteins (KASH proteins) at the nuclear envelope (NE), but their roles in nuclear positioning in mice are unknown. Here we show that the synaptic nuclear anchorage is partially perturbed in Sun1, but not in Sun2, knockout mice. Disruption of 3 or all 4 Sun1/2 wild-type alleles revealed a gene dosage effect on synaptic nuclear anchorage. The organization of nonsynaptic nuclei is disrupted in Sun1/2 double-knockout (DKO) mice as well. We further show that the localization of Syne-1 to the NE of muscle cells is disrupted in Sun1/2 DKO mice. These results clearly indicate that SUN1 and SUN2 function critically in skeletal muscle cells for Syne-1 localization at the NE, which is essential for proper myonuclear positioning.

 

韩珉

美国科罗拉多大学终身职位的教授。1983年起他在加洲大学洛杉叽分校师从著名分子遗传学家Michael Grunstein研究组蛋白功能，首次用遗传方法确立了组蛋白和核小体在活体转录调控和染色体分离中的功能。1986年和1988年作为研究生在美国生化协会大会和Gordon会议上发表演讲。研究成果后来在《纽约时报》和《科学美国人》中被描述。1988年起在加洲理工学院追随著名遗传学家Paul Sternberg作博士后。1990年确立了Ras原癌基因在发育模式形成中的功能,为此Cell和Nature杂志发表了专题综述文章。美国报界也广泛报道了这一结果。1991年起在科罗拉多大学任教。在使用抑制基因筛选Ras调控信号通路中的调控基因和线虫发育的多个研究领域作出了重要贡献。同时还参与人类疾病基因定位、克隆与研究。1997年成为著名休斯医学研究院成员。还先后获得美国生命科学研究基金会博士后奖学金，Lucile Markey学者，Searle学者，Basial Oconner学者等多项奖励。现任NIH基金评审委员会成员，吴瑞协会理事。1999年起每年为复旦大学生科院的研究生讲学，现任复旦大学特聘讲座教授。2001年起与许田和庄原教授在复旦大学建立了发育生物学研究所并共同主持工作。