裴端卿创建新型iPS文章登权威期刊

　　

生物通报道，中国科学院广州生物医药与健康研究院院长裴端卿领衔的研究小组创建了一类新的iPS细胞系，文章Generation of induced pluripotent stem cell lines from tibetan miniature pig发表在最新一期的《Journal of Biological Chemistry》上。

 

到目前为止，成功建立的iPS细胞系有来自小鼠的，大鼠的，人类的以及猴子的。尚没有其他物种的iPS细胞系成功建立，而近期，裴端卿教授打破了这一常规，他建立了西藏微型猪的iPS细胞系。

 

 

裴端卿研究小组从西藏微型猪的胚胎中分离成纤维细胞，再用逆转录病毒将转录因子导入成纤维细胞中，成功诱导了猪的iPS细胞系。

 

将慢病毒感染胚胎成纤维细胞16天后，裴端卿研究小组获得了iPS细胞，他们发现，相比小鼠和大鼠的iPS细胞，猪的iPS细胞的特征与人类的胚胎干细胞更吻合。

 

小鼠和大鼠的iPS系已经建立，并用于实验室的研究中，但是小鼠和大鼠的体型大小，生理学特性以及寿命都与人类有很大差异。相对而言，猪在生理上与人的差异要小些，建立猪的iPS系更有利于研究人类的疾病和建立有效的治疗模式。

（生物通 小茜）

生物通推荐原文检索：Generation of induced pluripotent stem cell lines from tibetan miniature pig

Miguel Angel Esteban, Jianyong Xu, Jiayin Yang, Meixiu Peng, Dajiang Qin, Wen Li, Zhuoxin Jiang, Jiekai Chen, Kang Deng, Mei Zhong, Jinglei Cai, Liangxue Lai, and Duanqing Pei1

From the GIBH, China

【Abstract】

Induced pluripotent stem cells (iPS) technology appears to be a general strategy to generate pluripotent stem cells from any given mammalian species. So far, iPS cells have been reported for mouse, human, rat and monkey. These four species also have established embryonic stem cell (ESC) lines which serve as gold standard for pluripotency comparisons. Attempts have been made to generate porcine ESC by various means without success. Here we report the successful generation of pluripotent stem cells from fibroblasts isolated from Tibetan miniature pig using a modified iPS protocol. The resulting iPS cell lines more closely resemble human ESC than cells from other species, have normal karyotype, stain positive for alkaline phosphatase, express high levels of ESC-like markers (Nanog, Rex1, Lin28, SSEA4), and can differentiate into teratomas composed of the three germ layers. Since porcine physiology closely resembles human, the iPS cells reported here provide an attractive model to study certain human diseases or assess therapeutical applications of iPS in a large animal model.

 

裴端卿

博士，研究员，中科院广州生物医药与健康研究院院长，华南干细胞与再生医学研究所PI

研究方向

蛋白质在正常与癌细胞里的运送机制

EGFR 的信号传导机制与肺癌

干细胞的全能性调控机制

 

教育

1991-1995 美国密西根大学博士后

1985-1991 美国宾西法尼亚大学博士

1980-1984 华中农学院学士

 

专业工作经历

2002-　　 清华大学教授

1996-2004 美国明尼苏达大学助理教授，副教授

1995-1996 美国密西根大学研究员

 

论文发表选录

Pei, D., Weiss, S.J. 1995. Furin-dependent Intracellular Activation of the Human Stromelysin-3 Zymogen. Nature. 375: 244-247.

Pei, D. 1999. Identification and characterization of the fifth member of the membrane-type matrix metalloproteinase MT5-MMP. J. Biol. Chem. 274:8925-8932.

Jiang, A., Lehti, K., Wang, X., Weiss, S.J., Keski-Oja, J., and Pei, D. 2001. Regulation of Membrane-Type Matrix Metalloproteinase 1 Activity by Dynamin-Mediated Endocytosis. Proc. Natl. Acad. Sci. 98: 13693-8.

Pan. G., Pei. D. 2005. The Stem Cell Pluripotency Factor Nanog Activates Transcription with Two Unusually Potent Subdomains at its C-terminus. J. Biol. Chem. 280:1401-1407.

Pei, D. 2005. Matrix Metalloproteases Target Protease-activated Receptors During Tumor Progression.CancerCell, 7: 207-8.