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生物通报道，中国科学院植物研究所分子发育生物学研究中心主任林金星研究员近期发表的钙调蛋白对植物胞外信号传递的研究被Nature China评为一周研究亮点。

　　

生物通报道，中国科学院植物研究所分子发育生物学研究中心主任，首席研究员林金星近期发表的钙调蛋白对植物胞外信号传递的研究被Nature China评为一周研究亮点。

 

钙调蛋白是由148个氨基酸残基组成的单链蛋白质,能够与钙结合在细胞内具有促进信息传递的功能，对细胞的生长和发育具有重要的作用。林教授认为在植物中钙调蛋白还可能参与胞外信号传递过程。

 

在本研究中林金星研究小组证实钙调蛋白对植物细胞的跨膜信号传递过程中具有重要的意义。研究小组在钙调蛋白上标记量子点（quantum dots）。研究发现量子点标记的钙调蛋白能与植物细胞的浆膜结合。通过投射电子显微技术研究者可以观察到植物细胞膜上的量子点钙调蛋白。

 

研究小组还用微电极来检测细胞膜上的钙离子流，结果发现植物细胞上的量子点钙调蛋白有增强钙离子流的作用。

（生物通 小茜）

 

生物通推荐原文摘要：Calmodulin binds to extracellular sites on the plasma membrane of plant cells and elicits a rise in intracellular calcium concentration

【Abstract】

Calmodulin (CaM) is a highly conserved intracellular calcium sensor. In plants, CaM also appears to be present in the apoplasm and application of exogenous CaM has been shown to influence a number of physiological functions as a polypeptide signal, however, the existence and localization of its corresponding apoplasmic binding sites remain controversial. To identify the site(s) of action, a CaM-conjugated quantum dot (QD) system was employed for single molecule level detection at the surface of plant cells. Using this approach, we show that QD-CaM binds selectively to sites on the outer surface of the plasma membrane which was further confirmed by high-resolution transmission electron microscopy. Measurements of Ca2+ fluxes across the plasma membrane, using ion-selective microelectrodes, demonstrated that exogenous CaM induces a net influx into protoplasts. Consistent with these flux studies, calcium-green dextran and FRET experiments confirmed that applied CaM/QD-CaM elicited an increase in cytoplasmic Ca2+ levels. These results support the hypothesis that apoplasmic CaM can act as a signaling agent. These findings are discussed in terms of CaM acting as an apoplasmic peptide ligand to mediate transmembrane signaling in the plant kingdom.

 

 林金星

博士，首席研究员。现任植物分子与发育生物学研究中心主任。

 　
研究方向
植物细胞与生殖生物学
　 　
　
承担项目
 
　 
近五年来，主持国际组织资助项目2项，从国外获得经费16.8万美元；主持中国科学院重点项目1项，国家自然科学基金项目3项; 另外，参加其他课题3项。 
  
　
论文论著
 
　
曾在澳大利亚国立大学、德国波恩大学、马普育种研究所、比利时安特卫普大学、剑桥大学植物系合作研究，先后在国内外发表论文70多篇，其中48篇在SCI收录刊物。
　
2009年

1. Tong Chen, Xiaoqin Wu, Yanmei Chen, Xiaojuan Li, Mei Huang, Maozhong Zheng, František Baluška, 
      Jozef Šamaj, Jinxing Lin *. 2009. Combined proteomic and cytological analysis of Ca2+–calmodulin
      regulation in Picea meyeri pollen tube growth. Plant Physiology 149: 1111–1126
　

2. Qinli Wang, Bo Chen, Peng Liu, Maozhong Zheng, Yuqing Wang, Sujuan Cui, Daye Sun, Xiaohong Fang,
    Chun-Ming Liu, William J. Lucas, Jinxing Lin*. 2009. Calmodulin binds to extracellular sites on the plasma
    membrane of plant cells and elicits a rise in intracellular calcium concentration. Journal of Biological
    Chemistry 10.1074/jbc.M808028200
　

3. Qiaoling Liu, Bo Chen, Qinli Wang, Xiaoli Shi, Zeyu Xiao, Jinxing Lin, Xiaohong Fang*. 2009. Carbon
   Nanotubes as Molecular Transporters for Walled Plant Cells. Nano Letters 9: 1007–1010
　

4. Yuhua Wang, Tong Chen, Chunyang Zhang, Huaiqing Hao, Peng Liu, Maozhong Zheng, František Baluška,
    Jozef Šama, Jinxing Lin*. 2009. Nitric oxide modulates the influx of extracellular Ca2+ and actin filament
    organization during cell wall construction in Pinus bungeana pollen tubes. New Phytologist doi:
    10.1111/j.1469-8137.2009.02820.x