北大特聘教授邓宏魁新文章解析干细胞技术

　　

生物通报道，北京大学生命科学院干细胞和再生医学实验室，北京大学深圳研究生院生化与生物技术学院化学基因组实验室，北京大学附属人民医院等处的研究者在新一期的《Stem Cell》上发表文章，文章标题为：Pluripotin Combined with Leukemia Inhibitory Factor Greatly Promotes the Derivation of Embryonic Stem Cell Lines from Refractory Strains。

 

文章通讯作者是北京大学干细胞研究中心的邓宏魁教授。邓宏魁教授去年在Cell子刊Cell Stem Cell上发表了两篇文章，11月，邓宏魁教授与北大的丁明孝教授带领研究小组改进了传统的iPS技术，将iPS的转化效率提高了100倍。其后，12月又在Cell Stem Cell上发表一篇文章，成功地将成年猕猴的纤维母细胞诱导成iPS细胞。

 

本次在Stem Cell上发表的文章是一篇技术性的介绍文章，主要讲述的是一种优化后的小鼠胚胎干细胞分离技术，研究小组在分离胚胎干细胞的过程中用白血病抑制因子（Leukemia inhibitory factor，LIF）处理，结果可以提高胚胎干细胞的分离效率。

（生物通 小茜）

 

生物通推荐原文检索：Pluripotin Combined with Leukemia Inhibitory Factor Greatly Promotes the Derivation of Embryonic Stem Cell Lines from Refractory Strains

【Abstract】

Most mouse embryonic stem (ES) cells are derived from a 129 or C57BL/6 background, whereas the derivation efficiency of ES cells is extremely low on certain refractory types of background for which ES cells are highly desired. Here we report an optimized, highly efficient protocol by combining pluripotin, a small molecule, and leukemia inhibitory factor (LIF) for the derivation of mouse ES cells. With this method, we successfully isolated ES cell lines from five strains of mice, with an efficiency of 57% for NOD-scid, 63% for SCID beige, 80% for CD-1, and 100% for two F1 strains from C57BL/6xCD-1. By tracking the Oct4-positive cells in the Oct4-green fluorescent protein embryos in the process of ES cell isolation, we found that pluripotin combined with LIF improved the efficiency of ES cell isolation by selectively maintaining the Oct4-positive cells in the outgrowth. To our knowledge, this is the first report of ES cells being efficiently derived from immunodeficient mice on refractory backgrounds (NOD-scid on a NOD background and SCID beige on a BALB/c background). STEM CELLS 2009;27:383–389