Cell Counting Kit-8 (CCK-8)

货号	品名	规格	价格(￥)
EC008	CCK-8	5ml(500T)	488

产品介绍：

Cell Counting Kit 8简称CCK-8试剂盒，是用于测定细胞增殖或毒性实验中活细胞数目的一种高灵敏度，无放射性的比色检测法。

CCK-8属于MTT的升级产品，CCK-8溶液可以直接加入到细胞样品中,不需要预配各种成分，检测快速，毒性非常低。CCK-8基于水溶性四唑盐WST-8。工作原理为：WST-8在电子耦合试剂存在的情况下，可以被线粒体内的脱氢酶还原生成橙黄色的甲臜染料。甲臜染料能够溶解在组织培养基中，与活细胞数量成正比。通过比色，可以动态地量化活细胞的数量，从而对细胞增殖或药物毒性进行检测本试剂盒通常用于贴壁或悬浮细胞的周期与凋亡检测。如果用于组织，则需要把组织消化成单细胞状态，再可以进行检测。

主要特点：

最快速(即开即用)、准确(很低毒性，稳定)的细胞增殖检测方法。

酚红和血清对CCK法的检测不会造成干扰。

细胞毒性非常低，加入WST-8显色后，可以在不同时间反复用酶标仪读数从而找到最佳测定时间。

应用本产品文献：
1.Journal of Dairy Science. 2019 Aug 22. Investigation and comparison of the anti-tumor activities of lactoferrin, α-lactalbumin, and β-lactoglobulin in A549, HT29, HepG2, and MDA231-LM2 tumor models.（乳铁蛋白、α-乳清蛋白和β-乳球蛋白的抗肿瘤活性研究）

2.American Journal of Physiology-Cell Physiology. 2019 Aug 14. Exosomes secreted from osteocalcin-overexpressed endothelial progenitor cells promoted endothelial cell angiogenesis.（内皮细胞血管生成研究）

3.Journal of Cellular and Molecular Medicine. 2019 Jul 14. Long non‐coding RNA NR2F1‐AS1 promoted proliferation and migration yet suppressed apoptosis of thyroid cancer cells through regulating miRNA‐338‐3p/CCND1 axis.（甲状腺癌细胞的增殖和迁移研究）

4.BioMed Research International. 2019 May 26. Galangin Suppresses Renal Inflammation via the Inhibition of NF-κB, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells.（高良姜素与乳腺癌细胞凋亡研究）

5.BMB Rep.. 2019 Apr 30. Insufficient radiofrequency ablation-induced autophagy contributes to the rapid progression of residual hepatocellular carcinoma through the HIF-1α/BNIP3 signaling pathway.（射频消融诱导的自噬不足和肝癌研究）

6.Journal of Cellular Physiology. 2019 Feb 14. MFGE8 protects against CCl4‐induced liver injury by reducing apoptosis and promoting proliferation of hepatocytes.（MFGE8与CCI4诱导的肝损伤的保护作用研究）

7.BMC Pulmonary Medicine . 2019 Feb 6. CD147 increases mucus secretion induced by cigarette smoke in COPD.

8.Pharmacology. 2018 Jun 7. Galangin Induces Apoptosis in MCF-7 Human Breast Cancer Cells Through Mitochondrial Pathway and Phosphatidylinositol 3-Kinase/Akt Inhibition.（高良姜素与肾脏炎症研究）

9.Biomedicine & Pharmacotherapy . 2017 Dec 14. Brevilin A induces apoptosis and autophagy of colon adenocarcinoma cell CT26 via mitochondrial pathway and PI3K/AKT/mTOR inactivation.

10.Biomedicine & Pharmacotherapy . 2017 Aug 11. Carapax Trionycis extracts inhibit fibrogenesis of activated hepatic stellate cells via TGF-β1/Smad and NFκB signaling.

11.Molecular medicine Reports. 2017 Aug 9.CD147 blockade as a potential and novel treatment of graft rejection.

12.Journal of Cancer Research and Clinical Oncology . 2017 Jul 7. α2,6-linked sialic acid serves as a high-affinity receptor for cancer oncolytic virotherapy with Newcastle disease virus.

13.PeerJ . 2017 May 16. Radiosensitization effect of hsa-miR-138-2-3p on human laryngeal cancer stem cells.

14.The American Journal of Chinese Medicine . 2017 Feb 24. Flavonoids and Tannins from Smilax china L. Rhizome Induce Apoptosis Via Mitochondrial Pathway and MDM2-p53 Signaling in Human Lung Adenocarcinoma Cells.

15.Oncol Lett. 2017 Feb 13. Expression and function of MutT homolog 1 in distinct subtypes of breast cancer.

16.Journal of Experimental & Clinical Cancer Research . 2017 Jan 16. CAPZA1 modulates EMT by regulating actin cytoskeleton remodelling in hepatocellular carcinoma.

17.Scientific Reports. 2016 Nov 21. N-linked glycosylation at Asn152 on CD147 affects protein folding and stability: promoting tumour metastasis in hepatocellular carcinoma.